Modeling inter- and intraspecific interaction of microorganisms in a batch culture experiment

Aim. To demonstrate the possibility of using a batch culture model to study interaction Salmonella Enteritidis and Salmonell Typhimurium, which are currently leading serotypes in etiology salmonellosis, an vitro experiment both among themselves combination with Yersinia pseudotuberculosis Shigella flexneri . Materials methods. An experimental microorganism associations was created by co-cultivation different strains LB broth. The stains stored museum G.P. Somov Research Institute Epidemiology Microbiology at temperature –80 °C were used. behavior under monitored for 24 h 37 on shaker. As control, growth curves all monoculture studied. first research stage involved culturing S. Typhimurium followed addition (reference strain ATCC 12022) second stage. Results. In control experiment, tested cultures quite similar. However, when grown associations, outgrew Enteritidis. A similar conducted three pathogens also revealed that, monoculture, exhibit no significant differences rates. Y. is significantly suppressed from onset observation during hour. Under these conditions, takes dominant position. Conclusion. Our results that can be used between types microorganisms, as well their intraspecific relationships. We assume observed microorganisms explained, certain extent, Gause’s law competitive exclusion. This principle implies two species competing same limited food source cannot coexist one ecological niche.